These Vegan Sourdough Starter Biscuits are delectable. They bring joy to my home. The biscuit-mojo comes from the use of vegan buttermilk & activated sourdough starter.
This is an easy overnight recipe. Most of the time making these from-scratch-biscuits is inactive. I love waking up and cutting last night’s dough into biscuits.
I love these with Amazing Creamy Vegan Biscuit Gravy!
Sourdough Starter
You will need a sourdough starter for this recipe. I made my starter back in September of 2020 and I wish I’d done it sooner. I typically bake with it once a week, storing it in the fridge to slow down the fermentation. We use the discard to make delicious skillet bread topped with za’atar, scallions, and sesame seeds. I love finding tasty ways to use things that might otherwise get thrown out.
I made my starter the easy, no-waste way. Specifically, I used a yeast packet instead of relying on wild yeast from the environment. I did not have to feed or dump any of the starter. I left it covered on the counter for up to 4 – 8 days (I’m not sure how long).
Now that my starter has been around for a while, I’d wager that the yeast in it is now wild yeast, from Colorado air. There is likely little difference between a starter made the dump-and-feed way. All starters get better with age.
I made my starter with the help of this youtube video from Allrecipes.com. If you are offended by the the yeast packet shortcut, there are plenty of other videos with different ways to make sourdough starter on youtube and all over the internet. If your starter turns orange or pink, please throw it out and start over.
Don’t do the twist
When cutting biscuits do not twist the biscuit cutter when cutting out the biscuits. The twisting motion will seal the edges, making it harder for them to rise.
Biscuit cutter options

Altitude
This recipe was adapted from a non-vegan recipe by Amy & Mike at Little Spoon Farm who live near the foothills of Tennessee mountains. I think it’s safe to assume it was not a high-altitude recipe. I’m in Denver, a mile high and I suspect this recipe adaptation works well in drier areas and varied altitudes.
Climate
When or baking in warm or high-humidity areas you may need increase the plant milk and bake a little longer. It make take a few batches to find the perfect balance for your neck of the woods.
Weigh or measure the flour
I use this food scale. If you don’t have a food scale, use a spoon to fluff up the flour in the bag. Then spoon the flour into a measuring cup until it is heaped on top. Level the top off with a butterknife.
No grater? No problem
If you don’t have a grater, the cold Earth Balance can be cut into small cubes, then use a pastry cutter or a fork to cut the Earth Balance into the flour. You can also use your hands to rub the Earth Balance cubes into the flour.
Cast iron skillet or baking sheet?
I prefer using a cast iron skillet or skillets, if I am baking all of the biscuits at once, instead of freezing half. However, a baking sheet works just fine. Either way, arrange the biscuits so there is about 1/4 – 1/2 inch of space between each biscuit.
Rise
Occasionally I get a low overnight rise and sometimes a very high overnight rise. This depends on how active the starter was and at what point during the rise I start cutting the biscuits. This stuff also depends on how warm it is, humidity and a bunch of other minutiae. For me, the biscuits turn out delicious. Sometimes they are fluffier (higher rise) and sometimes the are a little denser (lower rise).
Easy freeze biscuit dough
To freeze some or all of the biscuits, just cut the dough into biscuits and carefully place them in one layer, into a freezer bag or freezer-safe air-tight container. I bake them within a month’s time when I do this.

No need to thaw, just place them on to the skillet or baking sheet and bake from frozen. If using cast iron you may want to place a baking sheet under the cast iron skillet because sometimes frozen biscuits over cook on the bottom from frozen. For safety purposes, place the baking sheet in the oven first and then add the cast iron to it. Avoid lifting the baking sheet with the cast iron on top of the baking sheet.
Let’s Make Vegan Sourdough Starter Biscuits!
Please adjust the timeline on the recipe to your schedule.
Day 1
Noon: Activate starter, this usually takes 6 – 12 hours. To activate the starter add 1/4 cup water and 1/4 cup flour. You can also add a pinch of sugar to jumpstart activation, just don’t over do it. Stir and it will get bubbly and grow. The starter is ready to use when it’s 50% to 100% larger. Read more about this and how to do a spoon test here.
9 PM: Make the Vegan Buttermilk by stirring lemon juice or apple cider vinegar into the plant milk and letting it sit for 10 – 30 minutes. It will curdle.

Measure the flour into a mixing bowl using a zeroed-out food scale if you have one.

Grate the Earth Balance stick into the mixing bowl of flour.

Use a spatula or a fork to cut the cold Earth Balance into the flour until it forms small, pea-sized crumbles.
Add active sourdough starter, Vegan Buttermilk, salt & sugar and stir with a stiff spatula until fully combined.
Cover the bowl with plastic wrap, cheesecloth, or a thin, clean, non-fuzzy kitchen towel and let it sit on the counter for 10-12 hours.
Day 2:

9 AM: Generously flour your work surface and turn the dough out onto it.

Flour the top of the dough and use your hands to press it into an oval shape, about 1 inch in thickness.

Use a biscuit cutter (see notes for options) to cut biscuits, pressing straight down and pulling straight up. Do not twist the cutter as it will seal the edges and interfere with the biscuits ability to rise. Press down, then wiggle it a little if needed and pull straight up, just don’t twist.
Fold together and press out the remaining scrap dough and cut out one more round of biscuits.
Place the cut biscuits 1/4 – 1/2 inch apart in a cast iron skillet or on a baking sheet or combination of both. I usually cut all of the biscuits and put 6-7 in a ~12″ cast iron skillet and freeze the rest of the cut biscuits in a freezer bag. When I’m feeding more peeps I bake all of the biscuits.

Let the cut biscuits rise in the cast iron or on the baking sheets for 60 – 90 minutes. Preheat your oven to 400°F (204°C). I let them sit on the oven while it preheats.

Bake for 25-30 minutes or until the tops are golden. Serve & Enjoy.

Vegan Sourdough Starter Biscuits
Equipment
- oven
- 1 – 2 cast iron skillets or a baking sheet
- biscuit cutter or see alternatives in notes below
Ingredients
- 2 ½ cups 300 g all-purpose flour
- 1/2 cup 113 g Earth Balance cold
- ½ cup 125 g active sourdough starter see notes below on how to activate starter
- ¾ teaspoon salt fine
- 1 teaspoon sugar
Vegan Buttermilk
- 3/4 cup + 2 TBSPs ~210 grams oat or almond milk
- 1 TBSP lemon juice or apple cider vinegar
Instructions
Day 1
- Noon: Activate starter, this usually takes 6 – 12 hours. To activate the starter add 1/4 cup water and 1/4 cup flour. You can also add a pinch of sugar to jumpstart activation, just don't over do it. Stir and it will get bubbly and grow. The starter is ready to use when it's 50% to 100% larger. Read more about this and how to do a spoon test here.
- 9 PM: Make the Vegan Buttermilk by stirring lemon juice or apple cider vinegar into plant milk and letting it sit for 10 – 30 minutes. It will curdle.
- Measure the flour into a mixing bowl using a zeroed-out food scale if you have one. Grate the Earth Balance stick into the mixing bowl of flour.
- Use a spatula or a fork to cut the cold Earth Balance into the flour until it forms small, pea-sized crumbles.
- Add active sourdough starter, Vegan Buttermilk, salt & sugar and stir with a stiff spatula until fully combined. Cover the bowl with plastic wrap, cheesecloth, or a thin, clean, non-fuzzy kitchen towel and let it sit on the counter for 10-12 hours.
Day 2
- 9 AM: Generously flour your work surface and turn the dough out onto it.
- Flour the top of the dough and use your hands to press it into an oval shape, about 1 inch in thickness.
- Use a biscuit cutter (see notes for options) to cut biscuits, pressing straight down and pulling straight up. Do not twist the cutter as it will seal the edges and interfere with the biscuits ability to rise. Press down, then wiggle it a little if needed and pull straight up, just don't twist.
- Fold together and press out the remaining scrap dough and cut out one more round of biscuits.
- Place the cut biscuits 1/4 – 1/2 inch apart in a cast iron skillet or on a baking sheet or combination of both. I usually cut all of the biscuits and put 6-7 in a ~12" cast iron skillet and freeze the rest of the cut biscuits in a freezer bag. When I'm feeding more peeps I bake all of the biscuits.
- Let the cut biscuits rise in the cast iron or on the baking sheets for 60 – 90 minutes. Preheat your oven to 400°F (204°C). I let them sit on the oven while it preheats.
- Bake for 25-30 minutes or until the tops are golden. Serve & Enjoy.
Notes
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Recipe adapted from Amy & Mike at Little Spoon Farm.
If you enjoyed this recipe you might also enjoy
- Amazing Creamy Vegan Biscuit Gravy
- Addictive Coconut Bacon Flakes
- Vegan Tofu Veggie Scramble
- Oven-Baked Sriracha Tofu
- Vegan Garden Veggie Cream Cheese
Did You Make Vegan Sourdough Starter Biscuits?
How did it go? What did you eat your biscuits with? Tell me about it in the comments below. If this recipe went well for you, please leave a 5-star rating in the recipe card to help others find it.
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